Journal: Cell Death & Disease

Article Title: CUGBP1, a crucial factor for heart regeneration in mice

doi: 10.1038/s41419-022-04570-w

Figure Lengend Snippet: One-day ( A ) or 8-day ( B ) old mice were performed sham or apical resection surgery, CUGBP1 levels in cardiac tissue samples were detected by western blot analysis at 1,4,7,14 days post resection. Western blot bands were quantified by densitometry and normalized to GAPDH. dpr, days post resection. Data are the mean ± SEM ( n = 4–6). * p < 0.05, ** p < 0.01 vs. control.

Article Snippet: Briefly, antibody to CUGBP1 (Santa Cruz Biotechnology, cat# sc-20003), and IgG (Santa Cruz Biotechnology, cat# sc-2025) was added to supernatant for immunoprecipitation (IP) and mock group, respectively.

Techniques: Western Blot, Control

Journal: Cell Death & Disease

Article Title: CUGBP1, a crucial factor for heart regeneration in mice

doi: 10.1038/s41419-022-04570-w

Figure Lengend Snippet: A CUGBP1 was overexpressed in the 14-day old mice hearts and apical resection was performed simultaneously. Seven days after resection, cardiac tissue from apex zone was collected for paraffin section immunofluorescence detection of PH3 (red) and TNNI (green). Representative images are presented. B Statistical analysis of the number of PH3/TNNI-positive cells in apex and remote zone in the 14-day old mice heart following the Ad-virus infection and resection on seven days after resection. C Statistical analysis of the number of PH3/TNNI-positive cells by paraffin sections for immunofluorescence in the 14-day old mice heart following the Ad-virus infection and resection. Scale bars: 50 μm, 10 μm(inset). Data are presented as the mean ± SEM ( n = 8, 9 fields each mouse). * p < 0.05, ** p < 0.01 vs. control. dpi&r, days post injection & resection.

Article Snippet: Briefly, antibody to CUGBP1 (Santa Cruz Biotechnology, cat# sc-20003), and IgG (Santa Cruz Biotechnology, cat# sc-2025) was added to supernatant for immunoprecipitation (IP) and mock group, respectively.

Techniques: Paraffin Section, Immunofluorescence, Virus, Infection, Control, Injection

Journal: Cell Death & Disease

Article Title: CUGBP1, a crucial factor for heart regeneration in mice

doi: 10.1038/s41419-022-04570-w

Figure Lengend Snippet: A , B Cardiac tissues from 14-day old mice with CUGBP1 over-expression and apical resection were collected at 7 days after the surgery and mRNA expression level of Sfrp5 and Wif1 were determined by real-time quantitative PCR. C Western blotting of ki67 in Ad-virus infected primary cardiomyocyte with IMR treatment. Bands were quantified by densitometry and normalized to GAPDH. D Statistical analysis of western blot with GATA4 expression upon CUGBP1 inactivation in 1-day old mice heart. E Statistical analysis of western blot with GATA4 abundance upon CUGBP1 over-expression and IWR-1 treatment. Data are the mean ± SEM ( n = 3). * p < 0.05, ** p < 0.01 vs. control.

Article Snippet: Briefly, antibody to CUGBP1 (Santa Cruz Biotechnology, cat# sc-20003), and IgG (Santa Cruz Biotechnology, cat# sc-2025) was added to supernatant for immunoprecipitation (IP) and mock group, respectively.

Techniques: Over Expression, Expressing, Real-time Polymerase Chain Reaction, Western Blot, Virus, Infection, Control

Journal: Cell Death & Disease

Article Title: CUGBP1, a crucial factor for heart regeneration in mice

doi: 10.1038/s41419-022-04570-w

Figure Lengend Snippet: A RIP analysis was carried out using antibodies against CUGBP1 in NRVMs and RT-PCR assay was performed using primers targeting Wif1, Sfrp5, or GATA4, respectively. B Proposed mechanism of CUGBP1-Wnt/β-catenin-GATA4 regulatory axis. CUGBP1 abundance is upregulated upon apical resection. Therefore, altered levels of CUGBP1 interfere and downregulate the Sfrp5 and Wif1, which leads to activation of Wnt/β-catenin signaling. Consequently, cardiomyocyte proliferation and GATA4 levels is elevated, which could contribute to heart regeneration.

Article Snippet: Briefly, antibody to CUGBP1 (Santa Cruz Biotechnology, cat# sc-20003), and IgG (Santa Cruz Biotechnology, cat# sc-2025) was added to supernatant for immunoprecipitation (IP) and mock group, respectively.

Techniques: Reverse Transcription Polymerase Chain Reaction, Activation Assay